Whole exome capture in solution with 3 Gbp of data.
Matthew N Bainbridge,
Min Wang,
Daniel L Burgess,
Christie Kovar,
Matthew J Rodesch,
Mark D'Ascenzo,
Jacob Kitzman,
Yuan-Qing Wu,
Irene Newsham,
Todd A Richmond,
Jeffrey A Jeddeloh,
Donna Muzny,
Thomas J Albert and
Richard A Gibbs
Genome Biol 11(6):R62 (2010)
PMID 20565776
We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies.
DOI: 10.1186/gb-2010-11-6-r62
Version: za2963e q8za7 q8zbe q8zcd q8zd3 q8ze3 q8zf8 q8zg6
