Advanced search×

Isolation of human umbilical arterial smooth muscle cells (HUASMC).

Maximiano P MP Ribeiro, Ricardo R Relvas, Samuel S Chiquita, and Ilídio J IJ Correia

J Vis Exp (2010) PMID 20644508 PMCID PMC3156078

<object style="float: right;" type="application/x-shockwave-flash" data="http://www.jove.com/Resources/ThumbPlayer/ThumbPlayer_gray.swf?f=http://source.jove.com/1940_MINI.flv" width="204" height="156" wmode="opaque" onclick="window.open('http://www.jove.com/Details.php?ID=1940')" <param name="movie" value="http://www.jove.com/Resources/ThumbPlayer/ThumbPlayer_gray.swf?f=http://source.jove.com/XXXX_MINI.flv" /> <param name="wmode" value="opaque"> <!-- <p>Sorry, to view this content, you need the latest version of the Adobe Flash Player. Please download the<a href="http://get.adobe.com/flashplayer/"> Flash Player here</a>. Thank you.</p> --> </object>

The human umbilical cord (UC) is a biological sample that can be easily obtained just after birth. This biological sample is, most of the time, discarded and their collection does not imply any added risk to the newborn or mother s health. Moreover no ethical concerns are raised. The UC is composed by one vein and two arteries from which both endothelial cells (ECs) and smooth muscle cells (SMCs), two of the main cellular components of blood vessels, can be isolated. In this project the SMCs were obtained after enzymatic treatment of the UC arteries accordingly the experimental procedure previously described by Jaffe et al. After cell isolation they were kept in t-flash with DMEM-F12 supplemented with 5% of fetal bovine serum and were cultured for several passages. Cells maintained their morphological and other phenotypic characteristics in the different generations. The aim of this study was to isolate smooth muscle cells in order to use them as models for future assays with constrictor drugs, isolate and structurally characterize L-type calcium channels, to study cellular and molecular aspects of the vascular function and to use them in tissue engineering.

DOI: 10.3791/1940
Version: za2963e q8za6 q8zb5 q8zc3 q8zde q8ze7 q8zf5 q8zgf
View PDF

Similar articles you may find interesting…

  1. Moderate hypothermia attenuates oxidative stress injuries in alveolar epithelial A549 cells.
    Shr-Yun Chiou, Yu-Sheng Lee, and Wen-Jue Soong

    Exp Lung Res (2013) PMID 23647088

    We investigated the effects of hypothermia on ROS-induced cell damage in human alveolar type II cells. A549 cells were exposed to H2O2 and cultured at different temperatures, namely, normthermia (37°C), mild hypothermia (34°C), or moderate hypothermia (32°C). Cell damage was measured using variou...
    Buy PDF
  2. CD36 mediates endothelial dysfunction downstream of circulating factors induced by O3 exposure.
    Sarah Robertson, Elizabeth S Colombo, and Matthew J Campen

    Toxicol Sci (2013) PMID 23650127

    We hypothesized that CD36 plays a pivotal role in mediating cross-talk between inhaled ozone (O3)-induced circulating factors and systemic vascular dysfunction. O3 exposure (1 ppm x 4h) induced lung inflammation in WT mice that was absent in the CD36 deficient (CD36(-)/(-)) mice. Acetylcholine (ACh)...
    Buy PDF
  3. The metastatic microenvironment: Lung-derived factors control the viability of neuroblastoma lung metastasis.
    Shelly Maman, Liat Edry-Botzer, and Isaac P Witz

    Int J Cancer (2013) PMID 23649556

    We were able to generate two neuroblastoma cell populations - lung micrometastatic (MicroNB) cells and lung macrometastatic (MacroNB) cells. These two types of cells share the same genetic background, invade the same distant organ, but differ in their ability to create metastasis in the lungs. We hy...
    Buy PDF