The precise regulation of microtubules and their dynamics is critical for cell cycle progression, cell signaling, intracellular transport, cell polarization, and organismal development. For example, mitosis, cell migration, and axonal outgrowth all involve rapid and dramatic changes in microtubule organization and dynamics. Microtubule-associated proteins (MAPs) such as MAP2 and tau (Bunker et al., 2004; Dhamodharan and Wadsworth, 1995) and microtubule-interacting proteins such as stathmin, the kinesin MCAK, and EB1 (Cassimeris, 1999; Moore and Wordeman, 2004; Ringhoff and Cassimeris, 2009; Rusan et al., 2001) as well as numerous clinically approved or experimental anti-mitotic drugs including the taxanes, vinca alkaloids, and colchicine-like compounds modulate microtubule dynamic in cells (Jordan, 2002; Jordan and Kamath, 2007). In this chapter, we describe methods to analyze the dynamic instability of microtubules in living cells by microscopy of microinjected or expressed fluorescent tubulin, time-lapse microscopy, and analysis of time-dependent microtubule length changes.
We present Buddies,
The first systematic attempt to offer intersection attack resistant pseudonyms
In practical anonymity systems. Buddies groups users dynamically into buddy
Sets, controlling message transmission to make buddies within a set
Behaviorally indistinguishable to a traffic-monitoring ad...
We introduce a model for population
Dynamics under mutualism inspired by the logistic equation but avoiding
Singularities. The model is mathematically simpler than the widely used type II
Models, although it shows similar complexity in terms of fixed points and
Stability of the dynamics. Furthermore...
We treat the black hole horizon as a physical boundary to the
Spacetime and study its dynamics following from the Gibbons-Hawking-York
Boundary term. Using the Kerr black hole as an example we derive an effective
Action that describes, in the large wave number limit, a massless Klein-Gordon
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