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Isolation and characterization of microRNAs of human mature erythrocytes.

Carolyn Sangokoya, Gregory LaMonte, and Jen-Tsan Chi

Methods Mol Biol (2010) PMID 20827535

Human mature erythrocytes are terminally differentiated cells that have lost their nuclei and organelles during development. Even though mature erythrocytes lack ribosomal and other large-sized RNAs, they still retain small-sized RNAs. We have recently shown that there are abundant and diverse species of microRNAs in mature erythrocytes through the use of several different techniques, including northern blot, miRNA microarray, and real-time PCR. Furthermore, fractionation and genomic analysis has revealed that erythrocyte microRNA expression is different from that of reticulocytes or leukocytes and that mature erythrocytes contribute the majority of microRNA expression in whole blood. Therefore, global analysis of microRNA expression in circulating erythrocytes has the potential to provide mechanistic insights into erythrocyte biology and erythrocyte-related disorders. Here, we have provided the detailed methods for isolating and characterizing the microRNAs from human mature erythrocytes to enable such researches into human diseases involving erythrocytes.

DOI: 10.1007/978-1-60761-811-9_13
Version: za2963e q8za8 q8zbf q8zc3 q8zd8 q8ze5 q8zf5 q8zgc

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