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Centrifugal microfluidic system for primary amplification and secondary real-time PCR.

Maximilian M Focke, Fabian F Stumpf, Günter G Roth, Roland R Zengerle, and Felix F von Stetten

Lab Chip 10(23):3210-2 (2010) PMID 20938554

Pre-amplification is a basis for numerous polymerase chain reaction (PCR) protocols but bears severe contamination risks due to handling of high-copy DNA samples. Therefore we developed a self-contained centrifugal microfluidic system comprising pre-stored reagents; it enables pre-amplification of specific DNA sequences prior to automated aliquoting and real-time PCR in a modified commercial thermocycler.

DOI: 10.1039/c0lc00161a
Version: za2963e q8za4 q8zbf q8zce q8zd0 q8ze0 q8zfd q8zgc

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