Characterization and gene expression of transcription factors, PU.1 and C/EBP@a driving transcription from the tumor necrosis factor @a promoter in Japanese flounder, Paralichthys olivaceus
Both PU.1 and C/EBP@a transcription factors play important roles in myeloid development and inflammatory response. These transcripts were cloned from the Japanese flounder (Paralichthys olivaceus) and were highly conserved with those of other vertebrates. PU.1 mRNA was mainly expressed in lymphoid tissues while C/EBP@a mRNA was widely expressed in all tissues examined. Higher levels of PU.1 mRNA were expressed in the IgM^+ cells of both PBL and KL, while C/EBP@a expression was higher only in the IgM^- cells of KL. The expression of C/EBP@a mRNA was induced only in KL stimulated with LPS. Interestingly, PU.1 mRNA expression was induced by Edwardsiella tarda, whereas the expression of C/EBP@a mRNA was induced by Streptococcus iniae infection. Both PU.1 and C/EBP@a drove transcription from the LPS-responsive region of the Japanese flounder TNF@a gene, suggesting that both PU.1 and C/EBP@a induced by bacterial infection are involved in inflammation mediated through TNF@a expression.
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