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Isolation of photosystem II reaction center complexes from plants.

Michael Seibert and Rafael Picorel

Methods Mol Biol (2011) PMID 20960118

Methods to isolate and purify 6- and 5-Chl D1/D2/Cyt b559 photosystem II (PSII) reaction center (RC) complexes from plants are presented, and the advantages and disadvantages of each procedure are discussed. One of the simpler 6-Chl procedures and a procedure for isolating 5-Chl complexes are described in detail. Furthermore, a rapid procedure that produces relatively large amounts of less pure 6-Chl material (i.e., more nonpigmented protein) is also described. Criteria to assess the purity of PSII RC preparations are presented, and problems associated with each of the isolation procedures are discussed.

DOI: 10.1007/978-1-60761-925-3_3
Version: za2963e q8zaa q8zb4 q8zc3 q8zdc q8zec q8zfe q8zgd

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