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Isolation of ribulose-1,5-bisphosphate carboxylase/oxygenase from leaves.

A Elizabete Carmo-Silva, Csengele Barta, and Michael E Salvucci

Methods Mol Biol (2011) PMID 20960141

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a multifunctional enzyme that catalyzes the fixation of CO2 and O2 in photosynthesis and photorespiration, respectively. As the rate-limiting step in photosynthesis, improving the catalytic properties of Rubisco has long been viewed as a viable strategy for increasing plant productivity. Advances in biotechnology have made this goal more attainable by making it possible to modify Rubisco in planta. To properly evaluate the properties of Rubisco, it is necessary to isolate the enzyme in pure form. This chapter describes procedures for rapid and efficient purification of Rubisco from leaves of several species.

DOI: 10.1007/978-1-60761-925-3_26
Version: za2963e q8za6 q8zb2 q8zce q8zd9 q8ze4 q8zf9 q8zg5

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