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Clinical proteomics: liquid chromatography-mass spectrometry purification systems.

Michael Henry and Paula Meleady

Methods Mol Biol (2011) PMID 20978984

Liquid chromatography/mass spectrometry (LC/MS) has become a routine powerful technology in clinical proteomic studies in protein identification, protein characterisation, and the discovery of biomarkers. In this chapter, we describe simple protocols for protein digestion and the analysis of digested peptides produced both by one-dimensional reversed-phase liquid chromatography and two-dimensional liquid chromatography (i.e. strong cation exchange chromatography followed by reversed-phase chromatography), coupled with tandem mass spectrometry.

DOI: 10.1007/978-1-60761-913-0_28
Version: za2963e q8zad q8zb1 q8zc3 q8zda q8ze9 q8zf5 q8zge
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