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Identification of osteoclasts in culture.

Nobuyuki Udagawa, Teruhito Yamashita, Yasuhiro Kobayashi, and Naoyuki Takahashi

Methods Mol Biol (2011) PMID 21042999

Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte-macrophage lineage. Bone-forming osteoblasts play a role in the formation of osteoclasts. Osteoblasts/stromal cells express two cytokines essential for osteoclastogenesis: receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF). Using RANKL and M-CSF, osteoclasts can be induced from monocyte-macrophage lineage cells even in the absence of osteoblasts. We describe here methods for the identification of osteoclasts formed in vitro.

DOI: 10.1007/978-1-60761-962-8_18
Version: za2963e q8za9 q8zbc q8zc7 q8zd0 q8zea q8zf2 q8zgd
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