Advanced search×

Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.

Youngseon Choi, Junghan Lee, Keumhyun Kim, Heeyeon Kim, Peter Sommer, and Rita Song

Chem Commun (London) 46(48):9146-8 (2010) PMID 21049123

A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.

DOI: 10.1039/c0cc02702b
Version: za2963e q8za9 q8zb1 q8zc9 q8zd1 q8ze9 q8zfe q8zg3

Similar articles you may find interesting…

  1. Presence of Cryptosporidium spp. and Giardia duodenalis through drinking water
    José Antonio Castro-Hermida, Ignacio García-Presedo, ... Mercedes Mezo

    Sci Total Environ 405(1-3):9 (2008) PMID 18684490

    To evaluate the presence of Cryptosporidium spp. and Giardia duodenalis in the influent and final effluent of sixteen drinking water treatment plants located in a hydrographic basin in Galicia (NW Spain) - in which the principal river is recognised as a Site of Community Importance (SCI) - estimate...
  2. Giardia intestinalis genotypes: Risk factors and correlation with clinical symptoms
    A K Mohammed Mahdy, Johari Surin, ... Y A L Lim

    Acta Trop 112(1):4 (2009) PMID 19560431

    This study was conducted to identify genotypes related risk factors of Giardia intestinalis in an Orang Asli (aboriginal) community in Pahang, Malaysia. Stool samples were collected from 321 individuals aged between 2 and 76 years old, of whom 160 were males and 161 were females. Fae...
  3. Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission
    Lihua L Xiao and Ronald R Fayer

    Int J Parasitol 38(11):17 (2008) PMID 18479685

    The molecular characterisation of species and genotypes of Cryptosporidium and Giardia is essential for accurately identifying organisms and assessing zoonotic transmission. Results of recent molecular epidemiological studies strongly suggest that zoonotic transmission plays an important role in cry...