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Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase.

C S CS Pundir, Nidhi N Chauhan, and Jyoti

Artif Cells Blood Substit Immobil Biotechnol 39(3):177-84 (2011) PMID 21067286

Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin "Araldite" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R(2) = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C.

DOI: 10.3109/10731199.2010.525711
Version: za2963e q8za6 q8zb0 q8zc6 q8zdf q8ze4 q8zf5 q8zg4

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