Advanced search×

Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase.

C S CS Pundir, Nidhi N Chauhan, and Jyoti

Artif Cells Blood Substit Immobil Biotechnol 39(3):177-84 (2011) PMID 21067286

Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin "Araldite" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R(2) = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C.

DOI: 10.3109/10731199.2010.525711
Version: za2963e q8za6 q8zb0 q8zc6 q8zdf q8ze4 q8zf5 q8zg4
Buy PDF

Similar articles you may find interesting…

  1. Amperometric Biosensor based on Prussian Blue Nanoparticle-modified Screen Printed Electrode for Estimation of Glucose-6-phosphate.
    Suchanda Banerjee, Priyabrata Sarkar, and Anthony P F Turner

    Anal Biochem (2013) PMID 23648263

    We have reported a G6P biosensor based on screen-printed electrode utilizing Prussian Blue (PB) nanoparticles and enzymes, glucose-6-phosphate dehydrogenase and glutathione reductase. The PB nanoparticles acted as a mediator and thereby enhanced the rate of electron transfer in a bi-enzymatic reacti...
    Buy PDF
  2. A novel amperometric sensor for ascorbic acid based on poly(Nile blue A) and functionalised multi-walled carbon nanotube modified electrodes...
    Dilek Kul, M Emilia Ghica, and Christopher M A Brett

    Talanta (2013) PMID 23622528

    A new type of modified electrode sensor for ascorbic acid has been prepared by deposition of multi-walled carbon nanotubes (MWCNT) and poly(Nile blue A) on the surface of glassy carbon electrodes. Nile blue A was electropolymerised either beneath (directly on glassy carbon) or onto t...
    Buy PDF
  3. Effect of chlorpyrifos on the inhibition of the enzyme acetylcholinesterase by cross-linking in water-supply samples and milk from dairy cat...
    Diana Catalina Rodríguez, Stephanie Carvajal, and Gustavo Peñuela

    Talanta (2013) PMID 23622518

    A methodology for the determination of chlorpyrifos in water-supply samples and in milk from dairy cattle was developed. An amperometric biosensor was used to inhibit the enzyme acetylcholinesterase (AChE), which was immobilized by the cross-linking method (crosslinks between the enz...
    Buy PDF