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Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1.

M Stegger, J A Lindsay, A Moodley, R Skov, E M Broens, and L Guardabassi

J Clin Microbiol 49(2):732-4 (2011) PMID 21123532 PMCID PMC3043477

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

DOI: 10.1128/JCM.01970-10
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