Dynamic regulation of histone modifications is critical during development, and aberrant activity of chromatin-modifying enzymes has been associated with diseases such as cancer. Histone demethylases have been shown to play a key role in eukaryotic gene transcription; however, little is known about how their activities are coordinated in vivo to regulate specific biological processes. In Drosophila, two enzymes, dLsd1 (Drosophila ortholog of lysine-specific demethylase 1) and Lid (little imaginal discs), demethylate histone H3 at Lys 4 (H3K4), a residue whose methylation is associated with actively transcribed genes. Our studies show that compound mutation of Lid and dLsd1 results in increased H3K4 methylation levels. However, unexpectedly, Lid mutations strongly suppress dLsd1 mutant phenotypes. Investigation of the basis for this antagonism revealed that Lid opposes the functions of dLsd1 and the histone methyltransferase Su(var)3-9 in promoting heterochromatin spreading at heterochromatin-euchromatin boundaries. Moreover, our data reveal a novel role for dLsd1 in Notch signaling in Drosophila, and a complex network of interactions between dLsd1, Lid, and Notch signaling at euchromatic genes. These findings illustrate the complexity of functional interplay between histone demethylases in vivo, providing insights into the epigenetic regulation of heterochromatin/euchromatin boundaries by Lid and dLsd1 and showing their involvement in Notch pathway-specific control of gene expression in euchromatin.
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The introduction of an additional unknown variable to reduce the problem to
Solving a linear inequality, where the variable plays the role of a parameter.
A necessary and sufficient condition for the inequality to hold is used to
Evaluate the parameter, whereas the ge...
In this work, the effects of several technological factors on the stability of β-lapachone (βLAP) in solution and in the solid state were investigated.
The effects of relative humidity and light on the stability of βLAP in the solid state were studied. Samples were...
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This claim they first attempt to calculate the selective advantage of a local
Reduction in the mutation rate and conclude that it is not strong enough to be
Favoured by selection. Second, they analyse the distribution of 166 mut...
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