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Molecular Cloning and Sequence Analysis of Two Distinct Halotolerant Extracellular Proteases from Bacillus subtilis FP-133.

Shinji Takenaka, Nobutaka Yoshida, Ken-Ichi Yoshida, Shuichiro Murakami, and Kenji Aoki

Biosci Biotechnol Biochem 75(1):148-51 (2011) PMID 21228481

We cloned the genes encoding the two distinct extracellular halotolerant proteases of Bacillus subtilis FP-133 Expro-I and Expro-II, which were classified as alkaline serine and neutral proteases respectively. Three-dimensional modeling suggested that acidic and polar amino acid residues located on the surface stabilize protein structure in the presence of relatively high NaCl concentrations.

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