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Rapid characterization and engineering of natural product biosynthetic pathways via DNA assembler.

Zengyi Shao, Yunzi Luo, and Huimin Zhao

Mol Biosyst 7(4):1056-9 (2011) PMID 21327279

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

DOI: 10.1039/c0mb00338g
Version: za2963e q8za7 q8zb1 q8zcb q8zdb q8zee q8zf3 q8zgf

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