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Challenges and solutions for the identification of membrane proteins in non-model plants.

A A Vertommen, B B Panis, R R Swennen, and S C SC Carpentier

J Proteomics 74(8):1165-81 (2011) PMID 21354347

The workhorse for proteomics in non-model plants is classical two-dimensional electrophoresis, a combination of iso-electric focusing and SDS-PAGE. However, membrane proteins with multiple membrane spanning domains are hardly detected on classical 2-DE gels because of their low abundance and poor solubility in aqueous media. In the current review, solutions that have been proposed to handle these two problems in non-model plants are discussed. An overview of alternative techniques developed for membrane proteomics is provided together with a comparison of their strong and weak points. Subsequently, strengths and weaknesses of the different techniques and methods to evaluate the identification of membrane proteins are discussed. Finally, an overview of recent plant membrane proteome studies is provided with the used separation technique and the number of identified membrane proteins listed.

Copyright © 2011 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.jprot.2011.02.016
Version: za2963e q8za3 q8zbe q8zc1 q8zd6 q8ze3 q8zff q8zg3

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