DMAP1 (DNMT1-associated protein 1) is a member of the TIP60-p400 complex that maintains embryonic stem (ES) cell pluripotency and a complex containing the somatic form of DNA methyltransferase 1 (DNMT1s). DMAP1 interacts with DNMT1s through a domain that is absent in Dnmt1(V)(/)(V) mice expressing just the oocyte form (DNMT1o). A Dmap1-null allele was generated to study the role of DMAP1 in development. Consistent with the phenotypes of loss of other members of the TIP60-p400 complex, Dmap1(-/-) mice died during preimplantation in both Dnmt1(+/+) and Dnmt1(V)(/)(V) backgrounds. Unexpectedly, in the Dnmt1(V)(/)(V) background, Dmap1(+/-) parents produced mainly Dmap1(+/-) mice. Most Dmap1(+/+) progeny died during midgestation, with loss of DNA methylation on imprinted genes, suggesting that DMAP1 influences maintenance methylation mediated by DNMT1o. In this regard, a DMAP1-DNMT1o complex was detected in ES cells when DNMT1o was stably expressed but not when transiently expressed, indicating a novel interaction between DMAP1 and DNMT1o. These results suggest that DMAP1-DNMT1s and DMAP1-DNMT1o interactions are essential for normal development and that DMAP1-DNMT1o complexes are not readily formed in the embryo. Therefore, DMAP1 mediates distinct preimplantation epigenetic reprogramming processes: TIP60-p400 nucleosome remodeling and DNMT1 maintenance methylation.
Here the calculation of the loop-corrected trilinear NMSSM Higgs
Self-couplings. The loop corrections turn out to have a substantial impact on
The decay widths of Higgs-to-Higgs decays and on the production cross section
Of Higgs pairs via gluon fusion. They are therefore indispensable fo...
We develop a theory of fundamental strata for formal
Flat G-bundles. We show that any formal flat G-bundle contains a fundamental
Stratum; moreover, all such stratum have the same rational depth. We thus
Obtain a new invariant of a flat G-bundle called the slope, generalizing the
We use 2D hydrodynamical simulations to examine the role of
Planet mass on the gravitational stability of gaps and its impact on orbital
Migration. We consider giant planets with planet-to-star mass ratio q=0.0003 to
Q=0.003, in a self-gravitating disc with disc-to-star mass ratio M_d/M_*=0.08,
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