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Cloning and expression of a β-galactosidase gene of Bacillus circulans.

Jingyuan J Song, Hiroyuki H Imanaka, Koreyoshi K Imamura, Masashi M Minoda, Toru T Katase, Yukiko Y Hoshi, Shotaro S Yamaguchi, and Kazuhiro K Nakanishi

Biosci Biotechnol Biochem 75(6):1194-7 (2011) PMID 21670516

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.

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