Advanced search×

Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci.

Abdessalam A Cherkaoui, Stéphane S Emonet, José J Fernandez, Didier D Schorderet, and Jacques J Schrenzel

J Clin Microbiol 49(8):3004-5 (2011) PMID 21697322 PMCID PMC3147758

This study was undertaken to evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of beta-hemolytic streptococci. We compared Bruker Biotyper 2.0 with Vitek2 coupled to the agglutination test. MALDI-TOF MS analysis of 386 beta-hemolytic streptococcal isolates yielded high-confidence identification to the species level for all 386 isolates. The Vitek2 gave high-confidence identification to the species level for 88% of Streptococcus agalactiae isolates (n = 269/306), 92% of Streptococcus pyogenes isolates (n = 48/52), and 39% of isolates of Streptococcus dysgalactiae serogroups C and G (n = 11/28).

DOI: 10.1128/JCM.00240-11
Version: za2963e q8zac q8zba q8zc1 q8zd3 q8ze1 q8zfa q8zg9
View PDF

Similar articles you may find interesting…

  1. The CodY pleiotropic repressor controls virulence in gram-positive pathogens.
    Ludwig Stenz, Patrice Francois, ... Jacques Schrenzel

    FEMS Immunol Med Microbiol 62(2):123-39 (2011) PMID 21539625

    The highly conserved pleiotropic repressor CodY is involved in the post-exponential adaptive response to starvation in at least 30 different low G+C gram-positive bacteria. After dimerization and activation by co-factor binding, CodY binds to a consensus palindromic DNA sequence, lea...
  2. Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland.
    Laurent L Poirel, Jacques J Schrenzel, ... Patrice P Nordmann

    J Antimicrob Chemother 66(8):1730-3 (2011) PMID 21628303

    Objectives To analyse the mechanisms responsible for decreased susceptibility or resistance to carbapenems in several enterobacterial isolates recovered in 2009-10 in Geneva University Hospitals, Switzerland. Methods PCR and sequencing were used to identify β-lactamases, 16S RNA met...
  3. Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation.
    Qiang Q Lou, Tao T Zhu, ... Di D Qu

    BMC Microbiol (2011) PMID 21702925

    Deletion of saeRS in S. epidermidis resulted in an increase in biofilm-forming ability, which was associated with increased eDNA release and up-regulated Aap expression. The increased eDNA release from SE1457DeltasaeRS was associated with increased bacterial autolysis and decreased bacterial cell vi...
    View PDF