A long-sought milestone in microfluidics research has been the development of integrated technology for scalable analysis of transcription in single cells. Here we present a fully integrated microfluidic device capable of performing high-precision RT-qPCR measurements of gene expression from hundreds of single cells per run. Our device executes all steps of single-cell processing, including cell capture, cell lysis, reverse transcription, and quantitative PCR. In addition to higher throughput and reduced cost, we show that nanoliter volume processing reduced measurement noise, increased sensitivity, and provided single nucleotide specificity. We apply this technology to 3,300 single-cell measurements of (i) miRNA expression in K562 cells, (ii) coregulation of a miRNA and one of its target transcripts during differentiation in embryonic stem cells, and (iii) single nucleotide variant detection in primary lobular breast cancer cells. The core functionality established here provides the foundation from which a variety of on-chip single-cell transcription analyses will be developed.
We propose "Life-Add": a Lifetime Adjustable design for WiFi networks,
Where the devices are powered by battery, electric power, and/or renewable
Energy. In Life-Add, a device turns off its radio to save energy when the
Channel is sensed to be busy, and sleeps for a random time period before
We presented a long slit coronagraph (LSC), for
Which the presence of a slit in the coronagraphic focal plane induces a complex
Distribution of energy in the Lyot pupil-plane that cannot be easily masked
With a binary Lyot stop. To alleviate this concern, we propose to use a pupil
Apodization to sup...
We characterize different cell states, related to cancer and ageing
Phenotypes, by a measure of entropy of network ensembles, integrating gene
Expression values and protein interaction networks. The entropy measure
Estimates the parameter space available to the network ensemble, that can be
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