During infection of epithelial cells, the obligate intracellular pathogen Chlamydia trachomatis secretes the serine protease Chlamydia protease-like activity factor (CPAF) into the host cytosol to regulate a range of host cellular processes through targeted proteolysis. Here we report the development of an in vitro assay for the enzyme and the discovery of a cell-permeable CPAF zymogen-based peptide inhibitor with nanomolar inhibitory affinity. Treating C. trachomatis-infected HeLa cells with this inhibitor prevented CPAF cleavage of the intermediate filament vimentin and led to the loss of vimentin cage surrounding the intracellular vacuole. Because Chlamydia is a genetically intractable organism, this inhibitor may serve as a tool for understanding the role of CPAF in pathogenesis.
We have monitored 20 Sun-like stars in the Kepler field-of-view for excess
Flux with the FIES spectrograph on the Nordic Optical Telescope since the
Launch of Kepler spacecraft in 2009. These 20 stars were selected based on
Their asteroseismic properties to sample the parameter space (effective
We present deep Chandra
Observations of the cool core cluster Abell 2029, which has a sloshing spiral
Extending radially outward from the center of the cluster to approximately 400
Kpc at its fullest extent---the largest continuous spiral observed to date. We
Find a surface brightness excess, a temp...
We report on a Chandra/HETG X-ray spectrum of the transient X-ray binary MAXI
J1305-704. A rich absorption complex is detected in the Fe L band, including
Density-sensitive lines from Fe XX, XXI, and XXII. Spectral analysis over three
Wavelength bands with a large grid of XSTAR photoionization model...
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