Advanced search×

A Transcriptomic Atlas of Mouse Neocortical Layers

T Grant TG Belgard, Ana C AC Marques, Peter L PL Oliver, Hatice Ozel HO Abaan, Tamara M TM Sirey, Anna A Hoerder-Suabedissen, Fernando F García-Moreno, Zoltán Z Molnár, Elliott H EH Margulies, and Chris P CP Ponting

Neuron 71(4):12 (2011) PMID 21867878

In the mammalian cortex, neurons and glia form a patterned structure across six layers whose complex cytoarchitectonic arrangement is likely to contribute to cognition. We sequenced transcriptomes from layers 1-6b of different areas (primary and secondary) of the adult (postnatal day 56) mouse somatosensory cortex to understand the transcriptional levels and functional repertoires of coding and noncoding loci for cells constituting these layers. A total of 5,835 protein-coding genes and 66 noncoding RNA loci are differentially expressed (''patterned'') across the layers, on the basis of a machine-learning model (naive Bayes) approach. Layers 2-6b are each associated with specific functional and disease annotations that provide insights into their biological roles. This new resource (http://genserv.anat.ox.ac.uk/layers) greatly extends currently available resources, such as the Allen Mouse Brain Atlas and microarray data sets, by providing quantitative expression levels, by being genome-wide, by including novel loci, and by identifying candidate alternatively spliced transcripts that are differentially expressed across layers.

Copyright © 2011 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.neuron.2011.06.039
Version: za2963e q8zac q8zbd q8zc7 q8zde q8ze3 q8zf4 q8zg5

Similar articles you may find interesting…

  1. Differential expression of SNAP-25 family proteins in the mouse brain.
    Saori Yamamori, Makoto Itakura, ... Masami Takahashi

    J Comp Neurol 519(5):916-32 (2011) PMID 21280044

    We generated specific antibodies to distinguish these three homologous proteins. Immunoblot and immunohistochemical analyses revealed that SNAP-25b was distributed in synapse-enriched regions throughout almost the entire brain, whereas SNAP-25a and SNAP-23 were expressed in relatively specific brain...
  2. Developmental history of the subplate and developing white matter in the murine neocortex. Neuronal organization and relationship with the m...
    J A Del Río, A Martínez, ... E Soriano

    Cereb Cortex 10(8):784-801 (2000) PMID 10920050

    We conclude that the subplate neuronal population has a chemical heterogeneity reminiscent of that of the adult cortex and is crucial to the establishment of thalamocortical relationships, whereas the intermediate-subventricular neurons constituted a particular GABAergic population, which includes r...
    View PDF
  3. Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex.
    Joshua T JT Trachtenberg, Brian E BE Chen, ... Karel K Svoboda

    Nature 420(6917):788-94 (2002) PMID 12490942

    We repeatedly imaged individual pyramidal neurons in the mouse barrel cortex over periods of weeks. We found that, although dendritic structure is stable, some spines appear and disappear. Spine lifetimes vary greatly: stable spines, about 50% of the population, persist for at least a month, whereas...