Advanced search×

Sequence scrambling in shotgun proteomics is negligible.

Anton A AA Goloborodko, Mikhail V MV Gorshkov, David M DM Good, and Roman A RA Zubarev

J Am Soc Mass Spectrom 22(7):1121-4 (2011) PMID 21953093

Analysis of 15,897 low-energy (CAD) and 10,878 higher-energy (HCD) collisional dissociation mass spectra of doubly protonated tryptic peptides taken with high resolution revealed that the rate of sequence scrambling due to b-ion cyclization is negligible (

DOI: 10.1007/s13361-011-0130-z
Version: za2963e q8za7 q8zb1 q8zca q8zd8 q8zed q8zf4 q8zgf
Buy PDF

Similar articles you may find interesting…

  1. Proton-driven amide bond-cleavage pathways of gas-phase peptide ions lacking mobile protons.
    Benjamin J BJ Bythell, Sándor S Suhai, ... Béla B Paizs

    Audio, Transactions of the IRE Profes... 131(39):14057-65 (2009) PMID 19746933

    We describe the proton-driven amide bond cleavages necessary to produce b and y ions from peptide ions lacking easily mobilizable protons. We show that this important class of peptide ions fragments by different means from those with easily mobilizable protons. We present three new amide bond cleava...
    Buy PDF
  2. Effect of the basic residue on the energetics, dynamics, and mechanisms of gas-phase fragmentation of protonated peptides.
    Julia J Laskin, Zhibo Z Yang, ... Ivan K IK Chu

    J Am Chem Soc 132(45):16006-16 (2010) PMID 20977217

    The effect of the basic residue on the energetics, dynamics, and mechanisms of backbone fragmentation of protonated peptides was investigated. Time-resolved and collision energy-resolved surface-induced dissociation (SID) of singly protonated peptides with the N-terminal arginine res...
    Buy PDF
  3. The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.
    Hua H Jin, Susan Roehl SR White, ... Maxence V MV Nachury

    Cell 141(7):1208-19 (2010) PMID 20603001 PMCID PMC2898735

    We show that the BBSome constitutes a coat complex that sorts membrane proteins to primary cilia. The BBSome is the major effector of the Arf-like GTPase Arl6/BBS3, and the BBSome and GTP-bound Arl6 colocalize at ciliary punctae in an interdependent manner. Strikingly, Arl6(GTP)-mediated recruitment...
    View PDF