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Antigen-specific T cell phenotyping microarrays using grating coupled surface plasmon resonance imaging and surface plasmon coupled emission

Biosensors and Bioelectronics 31(1):264 (2012) PMID 22104646 PMCID PMC3249003

Highlights ► Characterization of circulating antigen-specific T lymphocytes represents a class of important biomarkers of immune function. ► We employ an SPR-based cellular microarray to detect antigen-specific T cells with a current limit of detection of 0.1%. ► After cell capture on the sensor chip, T cell functional status can be determined by measuring cytokine secretion patterns. ► The presence and functional status of T cell populations can be readily determined in a high-content SPR assay.

DOI: 10.1016/j.bios.2011.10.029