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Simultaneous saccharification and fermentation (SSF) of very high gravity (VHG) potato mash for the production of ethanol

Srichuwong, Fujiwara, Wang, Seyama, Shiroma, Arakane, Mukojima, and Tokuyasu

Biomass Bioenergy 33(5):9 (2009)

Simultaneous saccharification and fermentation (SSF) of very high gravity (VHG) potato mash, containing 304gL^-^1 of dissolved carbohydrates, was carried out for ethanol production. Potato tubers were ground into a mash, which was highly viscous. Mash viscosity was reduced by the pretreatment with mixed enzyme preparations of pectinase, cellulase and hemicellulase. The enzymatic pretreatment established the use of VHG mash with a suitable viscosity. Starch in the pretreated mash was liquefied to maltodextrins by the action of thermo-stable @a-amylase at 85^oC. SSF of liquefied mash was performed at 30^oC with the simultaneous addition of glucoamylase, yeast (Saccharomyces cerevisiae) and ammonium sulfate as a nitrogen source for the yeast. The optimal glucoamylase loading, ammonium sulfate concentration and fermentation time were 1.65AGUg^-^1, 30.2mM and 61.5h, respectively, obtained using the response surface methodology (RSM). Ammonium sulfate supplementation was necessary to avoid stuck fermentation under VHG condition. Using the optimized condition, ethanol yield of 16.61% (v/v) was achieved, which was equivalent to 89.7% of the theoretical yield.

Copyright © 2009 Elsevier Ltd. All rights reserved.

DOI: 10.1016/j.biombioe.2009.01.012
Version: za2963e q8zab q8zbd q8zcf q8zd1 q8zeb q8zf4 q8zg3

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