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  • UBE2S drives elongation of K11-linked ubiquitin chains by the Anaphase-Promoting Complex.
    We find that APC-catalyzed ubiquitination has an intrinsic preference for the K11 linkage of ubiquitin that is essential for substra...
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  • The Incoherent Feedforward Loop Can Provide Fold-Change Detection in Gene Regulation.
    We demonstrate theoretically that fold-change detection can be generated by one of the most common network motifs in transcription n...
    Mol Cell 36:894 (2009)
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  • Evidence that Fold-Change, and Not Absolute Level, of β-Catenin Dictates Wnt Signaling
    We found that the level of β-catenin is sensitive to perturbations in the pathway, such that cellular variation would be expected t...
    Mol Cell 36:872 (2009)
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  • Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling.
    We used a chemical genetic screen to identify a small molecule, XAV939, which selectively inhibits beta-catenin-mediated transcripti...
    Nature 461:614 (2009)
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  • Cell Growth and Size Homeostasis in Proliferating Animal Cells
    We examined cell size distributions in populations of lymphoblasts and applied a mathematical analysis to calculate how growth rates...
    Science 325:167 (2009)
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  • FLEXIQuant: a novel tool for the absolute quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides.
    We introduce a novel MS-based quantitative strategy, FLEXIQuant, (Full-Length Expressed Stable Isotope-labeled Proteins for Quantifi...
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  • The mechanism and pattern of yolk consumption provide insight into embryonic nutrition in Xenopus.
    We have gained insight into embryonic nutrition in the African clawed frog Xenopus laevis by studying YPs. Amphibians follow the anc...
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  • Large-scale detection of ubiquitination substrates using cell extracts and protein microarrays
    We have used extracts that replicate the mitotic checkpoint and anaphase release to identify differentially regulated poyubiquitinat...
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  • Different phosphorylation states of the anaphase promoting complex in response to antimitotic drugs: a quantitative proteomic analysis.
    We studied in detail the phosphorylation patterns at a single mitotic state of arrest generated by various antimitotic drugs. We exa...
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  • The relationship between evolutionary and physiological variation in hemoglobin.
    We use the hemoglobin molecule as a model system to quantify the relationship between physiological and evolutionary adaptations. We...
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  • The Drosophila homolog of MCPH1, a human microcephaly gene, is required for genomic stability in the early embryo.
    We identified mcph1, the Drosophila homolog of MCPH1, in a genetic screen for regulators of S-M cycles in the early embryo. Embryos...
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  • An actin-based wave generator organizes cell motility.
    We do not understand how these components act together to organize cell shape and movement. To address this question, we analyzed th...
    PLoS Biol 5:e221 (2007)
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  • Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities.
    We identified the deubiquitinating enzyme USP44 (ubiquitin-specific protease 44) as a critical regulator of the spindle checkpoint....
    Nature 446:876 (2007)
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  • Ubiquitination by the anaphase-promoting complex drives spindle checkpoint inactivation.
    We show that checkpoint inactivation is an energy-consuming process involving APC-dependent multi-ubiquitination. Multi-ubiquitinati...
    Nature 446:921 (2007)
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  • Regulation of Cdc25C by ERK-MAP kinases during the G2/M transition.
    We demonstrate that p42 MAP kinase (MAPK), the Xenopus ortholog of ERK2, is a major Cdc25 phosphorylating kinase in extracts of M ph...
    Cell 128:1119 (2007)
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  • Obituary: Reinhart Heinrich (1946-2006).
    Nature 444:700 (2006)
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  • Biochemical suppression of small-molecule inhibitors: a strategy to identify inhibitor targets and signaling pathway components.
    We report an approach for target identification and protein discovery based on functional suppression of chemical inhibition in vitr...
    Chem Biol 13:443 (2006)
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  • Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis.
    We characterize a family of complexes (which we term leading edge complexes), scaffolded by hematopoietic protein 1 (Hem-1), that or...
    PLoS Biol 4:e38 (2006)
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  • The processivity of multiubiquitination by the APC determines the order of substrate degradation.
    We show that substrate ordering depends on the relative processivity of substrate multiubiquitination by the APC. Processive substra...
    Cell 124:89 (2006)
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  • Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts.
    We have developed methods to study Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts. In this chapter, we describe...
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