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Phosphoinositides and membrane curvature switch the mode of actin polymerization via selective recruitment of toca-1 and Snx9.
Proc Natl Acad Sci U S A (2013) PMID 23589871
We show that curved membranes, but not flat ones, can use phosphatidylinositol 3-phosphate [PI(3)P] along with phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] to stimulate actin polymerization. In this case, actin polymerization requires the small GTPase cell cycle division 42 (Cdc42), the nucleat... -
Dynamics extracted from fixed cells reveal feedback linking cell growth to cell cycle.
Nature 494(7438):480-3 (2013) PMID 23446419
We describe a new method, ergodic rate analysis (ERA), that uses single-cell measurements of fixed steady-state populations to accurately infer the rates of molecular events, including rates of cell growth. ERA exploits the fact that the number of cells in a particular state is related to the averag... -
Mapping gene expression in two Xenopus species: evolutionary constraints and developmental flexibility.
Dev Cell 20(4):483-96 (2011) PMID 21497761
We examine Xenopus laevis and Xenopus tropicalis, two amphibians with very similar development, and ask how their transcriptomes compare. Despite separation for ~30-90 million years, there is strong conservation in gene expression in the vast majority of the expressed orthologs. Significant changes... -
Remodeling of the metabolome during early frog development.
PLoS One 6(2):e16881 (2011) PMID 21347444
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Dynamic regulation of the PR-Set7 histone methyltransferase is required for normal cell cycle progression.
Genes Dev 24(22):2531-42 (2010) PMID 20966048
We discovered the mechanisms that govern the dynamic regulation of PR-Set7 during mitosis, and that perturbation of these pathways results in defective mitotic progression. First, we found that PR-Set7 is phosphorylated at Ser 29 (S29) specifically by the cyclin-dependent kinase 1 (cdk1)/cyclinB com... -
Protein microarrays for genome-wide posttranslational modification analysis.
Wiley Interdiscip Rev Syst Biol Med 3(3):347-56 (2011) PMID 20865779
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Self-assembly of filopodia-like structures on supported lipid bilayers.
Science 329(5997):1341-5 (2010) PMID 20829485 PMCID PMC2982780
We have reconstituted the assembly of filopodia-like structures (FLSs). The actin assembles into parallel bundles, and known filopodial components localize to the tip and shaft. The filopodia tip complexes self-organize--they are not templated by preexisting membrane microdomains. The F-BAR domain p... -
Using buoyant mass to measure the growth of single cells.
Nat Methods 7(5):387-90 (2010) PMID 20383132 PMCID PMC2862099
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fractio... -
Quantitative Reconstitution of Mitotic CDK1 Activation in Somatic Cell Extracts
Mol Cell 37(6):15 (2010) PMID 20347419 PMCID PMC2882237
We have a quantitative understanding of this process. To gain a similar insight into somatic cells, we developed a human cell extract system that recapitulates CDK1 activation and nuclear envelope breakdown in response to mitotic cyclins. As cyclin B concentrations increase, CDK1 activates in a thre... -
Need-based up-regulation of protein levels in response to deletion of their duplicate genes.
PLoS Biol 8(3):e1000347 (2010) PMID 20361019
We address these questions at the genomic scale using high-throughput flow cytometry of single-cell protein levels in differentially labeled cocultures of wild-type and paralog-knockout Saccharomyces cerevisiae strains. We find that only a modest fraction of proteins (22 out of 202) show significant...
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