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  • Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste.
    Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin wa...
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  • Sticky PCR: A PCR-based protocol for targeted protein engineering
    This article describes a simple but powerful PCR-based protocol for the generation of cohesive ends on linear DNA fragments, permitti...
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  • Directed evolution of a Bacillus chitinase
    We established a straightforward directed evolution method for creating chitinase variants with improved properties. A library of mu...
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  • A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications
    We designed the triple-mutant T169G/E542K/V546C. Whereas the wild-type enzyme clearly favors D-glucose as its substrate over D-galac...
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  • A compact phage display human scFv library for selection of antibodies to a wide variety of antigens.
    Here we report the simplest and highly efficient method for the construction of a highly useful human single chain variable fragment...
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  • Mimotope identification from monoclonal antibodies of Burkholderia pseudomallei using random peptide phage libraries.
    These results demonstrate that phage-display technique has the potential for rapidly identifying phage mimotopes that interact with...
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  • Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei.
    Random peptide libraries displayed by bacteriophage T7 and M13 were employed to identify mimotopes from 4 monoclonal antibodies (MAb...
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  • Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia coli expression systems.
    We found that both the native signal peptides and that of E. coli outer membrane protein, OmpA, could be used to direct the secretio...
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  • Cloning and expression of the beta-galactosidase genes from Lactobacillus reuteri in Escherichia coli.
    Heterodimeric beta-galactosidase of Lactobacillus reuteri L103 is encoded by two overlapping genes, lacL and lacM. The lacL (1887bp)...
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  • Formation of galacto-oligosaccharides during lactose hydrolysis by a novel beta-galactosidase from the moderately thermophilic fungus Talaromyces thermophilus.
    Discontinuous and continuous processes of lactose hydrolysis and concomitant galacto-oligosaccharide (GalOS) formation were studied....
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  • A role for epsin N-terminal homology/AP180 N-terminal homology (ENTH/ANTH) domains in tubulin binding.
    We isolated and identified tubulin as an ENTH domain-binding partner. The interaction, which is direct and has a dissociation consta...
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  • Second cysteine-rich region of epidermal growth factor receptor contains targeting information for caveolae/rafts.
    We have used mutagenesis to determine that the region of the receptor that controls targeting to caveolae/rafts resides in the juxta...
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  • Screening phage-displayed combinatorial peptide libraries.
    Among the many techniques available to investigators interested in mapping protein-protein interactions is phage display. With a mod...
    Methods 24:240 (2001)
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  • Mapping protein-protein interactions with alkaline phosphatase fusion proteins.
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  • Molecular mechanism of NPF recognition by EH domains.
    I beta-turn motif. The residue directly following NPF is crucial for recognition and is required to complete the beta-turn. Five ami...
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  • Intersectin, an adaptor protein involved in clathrin-mediated endocytosis, activates mitogenic signaling pathways.
    We provide several lines of evidence that intersectin has the capacity to activate mitogenic signaling pathways. First, intersectin...
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  • The EH network.
    The EH domain is an evolutionary conserved protein-protein interaction domain present in a growing number of proteins from yeast to...
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  • Splice variants of intersectin are components of the endocytic machinery in neurons and nonneuronal cells.
    We recently identified and cloned intersectin, a protein containing two Eps15 homology (EH) domains and five Src homology 3 (SH3) do...
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  • Identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery.
    We have identified a approximately 140 amino acid domain that is shared by a variety of proteins in budding and fission yeast, nemat...
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  • Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains.
    We screened a Xenopus laevis oocyte cDNA expression library with a Src homology 3 (SH3) class II peptide ligand and identified a 127...
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