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High-content behavioral analysis of Caenorhabditis elegans in precise spatiotemporal chemical environments.
Nat Methods 8(7):599-605 (2011) PMID 21666667 PMCID PMC3152576
We describe a microfluidic system to analyze Caenorhabditis elegans behavior in defined temporal and spatial stimulus patterns. A 2 cm × 2 cm structured arena allowed C. elegans to perform crawling locomotion in a controlled liquid environment. We characterized behavioral responses to attractive odo... -
Catecholamine receptor polymorphisms affect decision-making in C. elegans.
Nature 472(7343):313-8 (2011) PMID 21412235
We have used quantitative genetic analysis to examine the decision to leave a food patch in Caenorhabditis elegans. Here we show that patch-leaving is a multigenic trait regulated in part by naturally occurring non-coding polymorphisms in tyra-3 (tyramine receptor 3), which encodes a G-protein-coupl... -
The homeodomain protein hmbx-1 maintains asymmetric gene expression in adult C. elegans olfactory neurons.
Genes Dev 24(16):1802-15 (2010) PMID 20713521 PMCID PMC2922507
We characterize these opposing influences on gene expression in Caenorhabditis elegans olfactory neurons. Using transcriptional reporters that are expressed differentially in two olfactory neurons, AWC(ON) and AWC(OFF), we identify mutations that affect the long-term maintenance of appropriate chemo... -
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
Nat Methods 6(12):875-881 (2009) PMID 19898485 PMCID PMC2858873
We developed a single-wavelength GCaMP2-based GECI (GCaMP3), with increased baseline fluorescence (3-fold), increased dynamic range (3-fold) and higher affinity for calcium (1.3-fold). We detected GCaMP3 fluorescence changes triggered by single action potentials in pyramidal cell dendrites, with sig... -
Wnt-Ror signaling to SIA and SIB neurons directs anterior axon guidance and nerve ring placement in C. elegans
Development 136(22):3801-3810 (2009) PMID 19855022
We demonstrate an essential role for Wnt signaling through Ror tyrosine kinase homologs in the most prominent anterior neuropil, the nerve ring. A genetic screen uncovered cwn-2, the C. elegans homolog of Wnt5, as a regulator of nerve ring placement. In cwn-2 mutants, all neuronal structures in and... -
Presynaptic CaV2 calcium channel traffic requires CALF-1 and the α2δ subunit UNC-36
Nat Neurosci 12(10):1257-1265 (2009) PMID 19718034 PMCID PMC2805665
We show here that a green fluorescent protein-tagged alpha(1) subunit of the Caenorhabditis elegans CaV2 channel, UNC-2, is localized to presynaptic active zones of sensory and motor neurons. Synaptic localization of CaV2 requires the alpha(2)delta subunit UNC-36 and CALF-1 (Calcium Channel Localiza... -
Neurons Detect Increases and Decreases in Oxygen Levels Using Distinct Guanylate Cyclases
Neuron 61(6):865-879 (2009) PMID 19323996 PMCID PMC2760494
We use behavioral analysis, functional imaging, and genetics to show that reciprocal changes in O(2) levels are encoded by sensory neurons that express alternative sets of sGCs. URX sensory neurons are activated by increases in O(2) levels, and require the sGCs gcy-35 and gcy-36. BAG sensory neurons... -
Quantitative Mapping of a Digenic Behavioral Trait Implicates Globin Variation in C. elegans Sensory Behaviors
Neuron 61(5):692-699 (2009) PMID 19285466 PMCID PMC2772867
We show that the C. elegans strains N2 and CB4856 have opposite behavioral responses to simultaneous changes in environmental O(2) and CO(2). We identify two quantitative trait loci (QTL) that affect this trait and map each QTL to a single-gene polymorphism. One gene, npr-1, encodes a previously des... -
Transcriptional regulation and stabilization of left-right neuronal identity in C. elegans.
Genes Dev 23(3):345-58 (2009) PMID 19204119
We show that the DNA-binding protein NSY-7 acts to convert a transient, partially differentiated state into a stable AWC(ON) identity. Expression of an AWC(ON) marker is initiated in nsy-7 loss-of-function mutants, but subsequently lost, so that most adult animals have two AWC(OFF) neurons and no AW... -
The Interscutularis Muscle Connectome
PLoS Biol 7(2):e32 (2009) PMID 19209956 PMCID 2637925
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