Latest papers

Elimination of broad-host range plasmid vectors in Escherichia coli by curring agents

CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis.

Substrate activity screening: a fragment-based method for the rapid identification of nonpeptidic protease inhibitors.

Characterization of the aminocoumarin ligase SimL from the simocyclinone pathway and tandem incubation with NovM,P,N from the novobiocin pathway.

Expression and characterization of the ATP-binding domain of a malarial Plasmodium vivax gene homologous to the B-subunit of the bacterial topoisomerase DNA gyrase.

Characterization of the formation of the pyrrole moiety during clorobiocin and coumermycin A1 biosynthesis.

Mass spectrometric pathway monitoring of secondary metabolites: systematic analysis of culture extracts of Streptomyces species.

Assembly of dimeric variants of coumermycins by tandem action of the four biosynthetic enzymes CouL, CouM, CouP, and NovN.

Fluoroquinolone-dependent DNA supercoiling by Vaccinia topoisomerase I.

Control of alpha subunit of eukaryotic translation initiation factor 2 (eIF2 alpha) phosphorylation by the human papillomavirus type 18 E6 oncoprotein: implications for eIF2 alpha-dependent gene expression and cell death.

Identification of a topoisomerase IV in actinobacteria: purification and characterization of ParYR and GyrBR from the coumermycin A1 producer Streptomyces rishiriensis DSM 40489.

In vitro and in vivo production of new aminocoumarins by a combined biochemical, genetic, and synthetic approach.

Reversal of breast cancer resistance protein (BCRP/ABCG2)-mediated drug resistance by novobiocin, a coumermycin antibiotic.

Membrane localization, oligomerization, and phosphorylation are required for optimal raf activation.

An unusual amide synthetase (CouL) from the coumermycin A1 biosynthetic gene cluster from Streptomyces rishiriensis DSM 40489.

CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis.

Agonist-independent desensitization and internalization of the human platelet-activating factor receptor by coumermycin-gyrase B-induced dimerization.

Transcriptional regulation of the ospAB and ospC promoters from Borrelia burgdorferi.

Resistance genes of aminocoumarin producers: two type II topoisomerase genes confer resistance against coumermycin A1 and clorobiocin.

groEL expression in gyrB mutants of Borrelia burgdorferi.