Latest papers

Ubiquitination of K-Ras enhances activation and facilitates binding to select downstream effectors.

Phage display: a powerful technology for the generation of high specificity affinity reagents from alternative immune sources.

Purification of proteins fused to maltose-binding protein.

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

Purification of antibodies using affinity chromatography.

Combination of chiroptical, absorption and fluorescence spectroscopic methods reveals multiple, hydrophobicity-driven human serum albumin binding of the antimalarial atovaquone and related hydroxynaphthoquinone compounds.

Proteomic analysis of Neorickettsia sennetsu surface-exposed proteins and porin activity of the major surface protein P51.

Expression and purification of two different antimicrobial peptides, PR-39 and Protegrin-1 in Escherichia coli.

Profiling the outer membrane proteome during growth and development of the social bacterium Myxococcus xanthus by selective biotinylation and analyses of outer membrane vesicles.

Intracellular protein glycosylation modulates insulin mediated lifespan in C.elegans.

Development of an efficient E. coli expression and purification system for a catalytically active, human Cullin3-RINGBox1 protein complex and elucidation of its quaternary structure with Keap1.

Glyceraldehyde-3-phosphate dehydrogenase as a surface associated antigen on human breast cancer cell lines MACL-1 and MGSO-3.

Chromatography of carbon nanotubes separated albumin from other serum proteins: a method for direct analysis of their interactions.

A rapid screening method for cell lines producing singly-tagged recombinant proteins using the TARGET tag system.

Disrupting 5-HT(2A) receptor/PDZ protein interactions reduces hyperalgesia and enhances SSRI efficacy in neuropathic pain.

Elastin-like polypeptides as a purification tag for recombinant proteins.

Proteomic complex detection using sedimentation (ProCoDeS): screening for proteins in stable complexes and their candidate interaction partners.

Large scale purification and characterization of recombinant human autotaxin/lysophospholipase D from mammalian cells.

Disrupting 5-HT(2A) receptor/PDZ protein interactions reduces hyperalgesia and enhances SSRI efficacy in neuropathic pain.

A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis.