Latest papers

Reflections on protein splicing: structures, functions and mechanisms.

PHB-intein-mediated protein purification strategy.

Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing.

Intein-mediated rapid purification of recombinant maxadilan and M65 and their acute effects on plasma glucose

[Preparation and identification of recombinant maxadilan]

Multiplex detection of protease activity with quantum dot nanosensors prepared by intein-mediated specific bioconjugation.

[Construction and evaluation of DnaB split intein high expression vector and a six amino acids cyclic peptide library].

Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan.

Single-process expression and purification of multiple recombinant proteins through cocultivation and affinity purification

Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags.

Site-specific chemical modification of proteins with a prelabelled cysteine tag using the artificially split Mxe GyrA intein.

Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction.

Toxic introns and parasitic intein in Coxiella burnetii: legacies of a promiscuous past.

Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system.

Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078: evolutionary relationships to phages from Corynebacterineae.

Phylogenetic analysis of PRP8 intein in Paracoccidioides brasiliensis species complex.

Divalent metal ions tune the self-splicing reaction of the yeast mitochondrial group II intron Sc.ai5gamma.

A circularly permuted beta-lactamase as a novel reporter for evaluation of protein cyclization efficiency.

Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage.

Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin