Latest papers

Activation of the ubiquitin-proteasome system in doxorubicin cardiomyopathy.

Proteomic and Functional Analyses Reveal a Dual Molecular Mechanism Underlying Arsenic-Induced Apoptosis in Human Multiple Myeloma Cells

In vitro assays for the anaphase-promoting complex/cyclosome (APC/C) in Xenopus egg extracts.

An in vitro assay for Cdc20-dependent mitotic anaphase-promoting complex activity from budding yeast.

Biochemical analysis of the Anaphase Promoting Complex: activities of E2 enzymes and substrate competitive (pseudosubstrate) inhibitors.

Preparation of synchronized human cell extracts to study ubiquitination and degradation.

Monoubiquitylation in the Fanconi anemia DNA damage response pathway.

Phosphorylation by Akt1 promotes cytoplasmic localization of Skp2 and impairs APCCdh1-mediated Skp2 destruction

Strong inducible knockdown of Cdc20 does not cause mitotic arrest in human somatic cells: implications for cancer therapy?

Strong inducible knockdown of APC/CCdc20 does not cause mitotic arrest in human somatic cells.

Checkpoint madness.

Engineering of the yeast ubiquitin ligase Rsp5: isolation of a new variant that induces constitutive inactivation of the general amino acid permease Gap1.

Lysine 63-linked polyubiquitin chain may serve as a targeting signal for the 26S proteasome

USP7 counteracts SCF TrCP- but not APCCdh1-mediated proteolysis of Claspin

Ubiquitin ligase Rsp5p is involved in the gene expression changes during nutrient limitation in Saccharomyces cerevisiae.

APC/C- and Mad2-mediated degradation of Cdc20 during spindle checkpoint activation.

Mechanisms of ubiquitin transfer by the anaphase-promoting complex.

Direct characterization of E2-dependent target specificity and processivity using an artificial p27-linker-E2 ubiquitination system.

Unexpected link between anaphase promoting complex and the toxicity of expanded polyglutamines expressed in yeast.

CaM kinase II initiates meiotic spindle depolymerization independently of APC/C activation.