1. Detection of ureolytic activity of bacterial strains isolated from entomopathogenic nematodes using infrared spectroscopy.

    Journal of Basic Microbiology 56(8):922 (2016) PMID 26972384

    The pathogenicity of entomopathogenic nematodes (EPNs) depends directly on the presence of bacteria in the nematode digestive tracts. Based on 16S rRNA and MALDI-TOF analyses 20 isolated bacteria were assigned to 10 species with 10 isolates classified as Pseudomonas ssp. Six strains (30%) show u...
  2. Application of Routine Diagnostic Procedure, VITEK 2 Compact, MALDI-TOF MS, and PCR Assays in Identification Procedure of Bacterial Strain with Ambiguous Phenotype.

    Current Microbiology 72(5):570 (2016) PMID 26804795

    In diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. A broad choice of methods is available, based on both phenotypic and molecular properties of microbes. The aim of this study was to compare the application of...
  3. Isolation of Staphylococcus microti from milk of dairy cows with mastitis.

    Veterinary Microbiology 182:163 (2016) PMID 26711044

    The present paper is a case-report of multiple udder infections in a dairy herd caused by Staphylococcus microti. Over a 22-month period, eleven S. microti isolates from milk samples from 9 cows were collected. The animals experienced subclinical (with one exception) intramammary infections with...
  4. Antibodies against Escherichia coli O24 and O56 O-Specific Polysaccharides Recognize Epitopes in Human Glandular Epithelium and Nervous Tissue.

    PLoS ONE 10(6):e0129492 (2015) PMID 26086646 PMCID PMC4472344

    Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, contains the O-polysaccharide, which is important to classify bacteria into different O-serological types within species. The O-polysaccharides of serotypes O24 and O56 of E. coli contain sialic acid i...
  5. Reptiles as a source of Salmonella O48--clinically important bacteria for children: the relationship between resistance to normal cord serum and outer membrane protein patterns.

    Microbial Ecology 61(1):41 (2011) PMID 20480364

    Bacteria of the Salmonella O48 somatic antigen group are clinically important strains causing intestinal dysfunction and diarrhoea, especially in children. The susceptibility of Salmonella O48 strains containing sialic acid (N-acetylneuraminic acid (NeuAc)) in lipopolysaccharide (LPS) to the bac...
  6. Structure of an abequose-containing O-polysaccharide from Citrobacter freundii O22 strain PCM 1555.

    Carbohydrate Research 344(13):1724 (2009) PMID 19576576

    The lipopolysaccharide of Citrobacter freundii O22 (strain PCM 1555) was degraded under mild acidic conditions and the O-polysaccharide released was isolated by gel chromatography. Sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H ROES...
  7. Structure of the glycerol phosphate-containing O-specific polysaccharide and serological studies on the lipopolysaccharides of Citrobacter werkmanii PCM 1548 and PCM 1549 (serogroup O14).

    FEMS Immunology and Medical Microbiology 54(2):255 (2008) PMID 18811720

    The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of Citrobacter werkmanii PCM 1548 and PCM 1549 (serogroup O14) and found to contain D-glucose, D-glucosamine and glycerol-1-phosphate in molar ratios 2 : 2 : 1. Based on methylation analysis and 1H and 1...
  8. Structure of a phosphoethanolamine-containing O-polysaccharide of Citrobacter freundii strain PCM 1443 from serogroup O39 and its relatedness to the Klebsiella pneumoniae O1 polysaccharide.

    FEMS Immunology and Medical Microbiology 53(1):60 (2008) PMID 18371072

    Lipopolysaccharide was extracted from cells of Citrobacter freundii PCM 1443 from serogroup O39 and degraded by mild acid hydrolysis to give an O-polysaccharide. Based on enzymatic and methylation analyses, along with 1H and 13C nuclear magnetic resonance spectroscopy, it was found that the lipo...
  9. Immunochemical studies of the lipopolysaccharides of Hafnia alvei PCM 1219 and other strains with the O-antigens containing D-glucose 1-phosphate and 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose.

    Archivum Immunologiae et Therapiae Experimentalis 56(5):347 (2008) PMID 18836891 PMCID PMC2768797

    Hafnia alveiis the only species of the genus Hafnia, which belongs to the family of Enterobacteriaceae. These Gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. Their lipopolysaccharides (LPSs) are important surfa...
  10. Structure of the O-polysaccharide of Hafnia alvei strain PCM 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation.

    Carbohydrate Research 340(2):263 (2005) PMID 15639246

    The O-polysaccharide of Hafnia alvei PCM 1189 consists of D-glucose, D-galactose, D-GalNAc and D-GlcA and lacks the strict regularity. The intact and carboxyl-reduced polysaccharides as well as oligosaccharides obtained by partial acid hydrolysis were studied by chemical and enzymatic analyses, ...
  11. Structure of the O-polysaccharide ofHafnia alveistrain PCM 1189 that has hexa- to octasaccharide repeating units owing to incomplete glucosylation

    Carbohydrate Research 340(2):263 (2005)

  12. [Bactericidal activity of normal human serum to gram-negative rods with sialic acid containing lipopolysaccharide].

    Medycyna doswiadczalna i mikrobiologia 56(3):255 (2004) PMID 15773502

    The complement plays the most important role in eliminating bacterial invasion of the host, by facilitating phagocytosis of potential pathogens and by participating in the direct essential role in protecting gram-negative bacteria against bactericidal activity of serum. Sialic acids which are im...
  13. Substitution pattern of 3-deoxy-D-manno-oct-2-ulosonic acid in bacterial lipopolysaccharides investigated by methylation analysis of whole LPS.

    Carbohydrate Research 338(23):2679 (2003) PMID 14670726

    3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) is a constituent of the inner core part of bacterial lipopolysaccharides (LPS). This sugar may contribute to biological activities of the LPS, the type of substitution of Kdo is thus of importance and this work is aimed at the evaluation of a method for ...
  14. Structure of the O-polysaccharide from the lipopolysaccharide of Hafnia alvei strain PCM 1546.

    Carbohydrate Research 338(20):2153 (2003) PMID 14505884

    An acidic O-polysaccharide isolated by mild acid hydrolysis from the lipopolysaccharide of Hafnia alvei PCM 1546 is composed of D-Gal, D-Glc, D-GlcA, D-GalNAc and O-acetyl groups in the ratios 1:1:1:2:1.6. On the basis of sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectro...
  15. Structural analysis of the Lactobacillus rhamnosus strain KL37C exopolysaccharide.

    Carbohydrate Research 338(7):605 (2003) PMID 12644373

    The exopolysaccharide from the lactic acid bacterium Lactobacillus rhamnosus strain KL37C isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. The polysaccharide mat...
  16. Substitution pattern of 3-deoxy-d-manno-oct-2-ulosonic acid in bacterial lipopolysaccharides investigated by methylation analysis of whole LPS

    Carbohydrate Research 338(23):2679 (2003)

    3-Deoxy- d- manno-oct-2-ulosonic acid (Kdo) is a constituent of the inner core part of bacterial lipopolysaccharides (LPS). This sugar may contribute to biological activities of the LPS, the type of substitution of Kdo is thus of importance and this work is aimed at the evaluati...
  17. Structural analysis of theLactobacillus rhamnosusstrain KL37C exopolysaccharide

    Carbohydrate Research 338(7):605 (2003)

    The exopolysaccharide from the lactic acid bacterium Lactobacillus rhamnosus strain KL37C isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. The polysa...
  18. Structure of a new 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose-containing O-specific polysaccharide from the lipopolysaccharide of Citrobacter gillenii PCM 1542.

    Carbohydrate Research 337(17):1541 (2002) PMID 12350323

    The O-specific polysaccharide of Citrobacter gillenii PCM 1542 from serotype O-12a,12 b is composed of one residue each of D-glucose, D-GlcNAc, 2-deoxy-2-[(R)-3-hydroxybutyramido]-D-glucose (D-GlcNAcyl) and two GalNAc residues. On the basis of sugar and methylation analyses of the intact and Smi...
  19. Structure of a new 2-deoxy-2-[(R)-3-hydroxybutyramido]-d-glucose-containing O-specific polysaccharide from the lipopolysaccharide ofCitrobacter gilleniiPCM 1542

    Carbohydrate Research 337(17):1541 (2002)

    The O-specific polysaccharide of Citrobacter gillenii PCM 1542 from serotype O-12a,12b is composed of one residue each of d-glucose, d-GlcNAc, 2-deoxy-2-[( R)-3-hydroxybutyramido]- d-glucose ( d-GlcNAcyl) and two GalNAc residues. On the basis of sugar ...