1. Upconverting nanoparticles: assessing the toxicity.

    Chemical Society Reviews 44(6):1561 (2015) PMID 25176037

    Lanthanide doped nanoparticles (Ln:NPs) hold promise as novel luminescent probes for numerous applications in nanobiophotonics. Despite excellent photostability, narrowband photoluminescence, efficient anti-Stokes emission and long luminescence lifetimes, which are needed to meet the requirement...
  2. Presumable role of outer membrane proteins of Salmonella containing sialylated lipopolysaccharides serovar Ngozi, sv. Isaszeg and subspecies arizonae in determining susceptibility to human serum.

    Gut Pathogens 7:18 (2015) PMID 26185527 PMCID PMC4504086

    The O48 group comprises Salmonella bacteria containing sialic acid in the lipopolysaccharide (LPS). Bacteria with sialylated surface structures are described as pathogens that avoid immunological response of the host by making similar their surface antigens to the host's tissues (molecular mimic...
  3. Application of quartz tuning forks for detection of endotoxins and Gram-negative bacterial cells by monitoring of Limulus Amebocyte Lysate coagulation.

    Biosensors and Bioelectronics 58:132 (2014) PMID 24632139

    Endotoxins, pyrogens of bacterial origin, are a significant threat in many areas of life. Currently, the test most commonly used for endotoxin level determination is LAL (Limulus Amebocyte Lysate) assay. This paper presents application of commercially available low-frequency piezoelectric tuning...
  4. Microcantilever array biosensors for detection and recognition of Gram-negative bacterial endotoxins

    Sensors and Actuators B: Chemical 198:114 (2014)

    • The use of cantilever sensors to detect bacterial endotoxins is proposed. • Specific antibodies are grown and tested using SDS-PAGE and ELISA methods. ...
  5. Preliminary study on application of urine amino acids profiling for monitoring of renal tubular injury using GLC-MS.

    Postepy higieny i medycyny doswiadczalnej (Online) 68(0):1299 (2014) PMID 25404619

    The early diagnosis of the nephrotoxic effect of xenobiotics and drugs is still an unsolved problem. Recent studies suggest a correlation between the nephrotoxic activity of xenobiotics and increased concentration of amino acids in urine. The presented study was focused on the application of GLC...
  6. H-Ras increases release of sphingosine resulting in down-regulation of TSP-1 in non-transformed cells.

    International Journal of Experimental Pathology 93(3):202 (2012) PMID 22356213 PMCID PMC3385918

    Tumour progression is continuously driven by a sequence of genetic events. The presence of mutant or activated Ras proteins represents an interesting paradigm for the investigation of oncogene-dependent induction of tumour angiogenesis. These genes are widely distributed in human cancers. Previo...
  7. Targeted Protein Immobilization on Si/Au Surfaces for Selective Functionalization of Si/SiO2Microcantilevers with Au Layer

    Procedia Engineering 47:1378 (2012)

    For cantilever sensors, working in deflection mode, targeted functionalization of selected side of cantilever is of great importance for many biochemical and medical applications. To find the optimal method of functionalization of sensitive and reference cantilever pair, we compared se...
  8. Structural studies of the exopolysaccharide consisting of a nonasaccharide repeating unit isolated from Lactobacillus rhamnosus KL37B.

    Carbohydrate Research 346(18):2926 (2011) PMID 22063501

    A novel structure of exopolysaccharide from the lactic acid bacteria (LAB) Lactobacillus rhamnosus KL37B, from the human intestinal flora, is described. During the structural investigation of the exopolysaccharide it was found that the repeating unit is a nonasaccharide, which is the largest rep...
  9. [Enterobacteriaceae infection - diagnosis, antibiotic resistance and prevention].

    Postepy higieny i medycyny doswiadczalnej (Online) 65:55 (2011) PMID 21357995

    Intestinal infections caused by rod-shaped bacteria of the Enterobacteriaceae genus are one of the major health hazards in countries where sanitation standards are low. Strains of Shigella, Salmonella, Escherichia and Yersinia are responsible for diarrhea, severe bacillary dysentery, typhoid, ot...
  10. Structural studies of the exopolysaccharide consisting of a nonasaccharide repeating unit isolated fromLactobacillus rhamnosusKL37B

    Carbohydrate Research 346(18):2926 (2011) PMID 22063501

    During the structural investigation of the exopolysaccharide it was found that the repeating unit is a nonasaccharide, which is the largest repeating unit found in LAB exopolysaccharides so far. Chemical structure of the EPS isolated from Lactobacillus rhamnosus KL37B:
  11. Sialic acid-containing lipopolysaccharides of Salmonella O48 strains--potential role in camouflage and susceptibility to the bactericidal effect of normal human serum.

    Microbial Ecology 59(3):601 (2010) PMID 19844648

    Sialic acid (N-acetylneuraminic acid, NeuAc) plays an essential role in protecting gram-negative bacteria against the bactericidal activity of serum and may contribute to the pathogenicity of bacteria by mimicking epitopes that resemble host tissue components (molecular mimicry). The role of sia...
  12. Structural and immunochemical studies of neutral exopolysaccharide produced by Lactobacillus johnsonii 142.

    Carbohydrate Research 345(1):108 (2010) PMID 19897181

    This paper describes the structure of neutral exopolysaccharide (EPS) produced by Lactobacillus johnsonii 142, strain of the lactic acid bacteria isolated from the intestine of mice with experimentally induced inflammatory bowel disease (IBD). Sugar and methylation analyses along with (1)H and (...
  13. Structure of the glycerol phosphate-containing O-specific polysaccharide and serological studies on the lipopolysaccharides of Citrobacter werkmanii PCM 1548 and PCM 1549 (serogroup O14).

    FEMS Immunology and Medical Microbiology 54(2):255 (2008) PMID 18811720

    The O-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of Citrobacter werkmanii PCM 1548 and PCM 1549 (serogroup O14) and found to contain D-glucose, D-glucosamine and glycerol-1-phosphate in molar ratios 2 : 2 : 1. Based on methylation analysis and 1H and 1...
  14. Application of supercritical carbon dioxide (scCO2) for the extraction of lipopolysaccharides (LPS) fromSalmonella entericasubsp.entericaPCM 2266

    The Journal of Supercritical Fluids 45(1):51 (2008)

    The paper describes a novel extraction procedure for lipopolysaccharides (LPS) from Salmonella enterica subsp. enterica (PCM 2266). Process parameters for the extraction of LPS from bacterial mass were optimized by carrying out a two-level fractional design experiment. Four pa...
  15. [Exopolysaccharides of lactic acid bacteria: structure and biosynthesis].

    Postepy higieny i medycyny doswiadczalnej (Online) 61:805 (2007) PMID 18097339

    The group of lactic acid bacteria (LABs) includes four genera: Lactobacillus, Leuconostoc, Pediococcus, and Streptococcus. The most characteristic feature of this group of microorganisms is the production of lactic acid as a main product of carbohydrate metabolism. LABs are responsible for the f...
  16. Biosensor applications of luminescence depolarization effects in protein-modified silica films doped with organic luminophores

    Journal of Luminescence 122:987 (2007)

    Thin films of silica functionalized with NH 2 groups were prepared by the sol–gel technique from tetraethoxysilane (TEOS) and (3-aminopropyl)-triethoxysilane (APTES) precursors. Selected proteins labeled with isothiocyanate fuorescein (FTIC) were covalently attached to their surfaces ...
  17. [Glycosaminoglycans in the pathogenesis and diagnostics of Graves's ophthalmopathy].

    Postepy higieny i medycyny doswiadczalnej (Online) 60:370 (2006) PMID 16885907

    Graves's ophthalmopathy is an extrathyroidal tissue-specific symptom of Graves's disease characterized by inflammatory infiltration of orbital tissues. In several percent of patients the course of disease is severe, leading to serious ocular complications and affecting quality of life. Progress ...
  18. Substitution pattern of 3-deoxy-D-manno-oct-2-ulosonic acid in bacterial lipopolysaccharides investigated by methylation analysis of whole LPS.

    Carbohydrate Research 338(23):2679 (2003) PMID 14670726

    3-Deoxy-D-manno-oct-2-ulosonic acid (Kdo) is a constituent of the inner core part of bacterial lipopolysaccharides (LPS). This sugar may contribute to biological activities of the LPS, the type of substitution of Kdo is thus of importance and this work is aimed at the evaluation of a method for ...
  19. Structure of the O-specific polysaccharide from the lipopolysaccharide of Citrobacter gillenii O11, strain PCM 1540.

    Carbohydrate Research 338(13):1389 (2003) PMID 12801712

    The O-specific polysaccharide of the lipopolysaccharide of Citrobacter gillenii PCM 1540 (serogroup O11) consists of D-Glc, D-Man, D-GalNAc, D-GlcNAc, 2-acetamido-2,6-dideoxy-D-galactose (D-FucNAc) and O-acetyl groups in the ratios 2:1:1:1:1:1. On the basis of sugar and methylation analyses and ...
  20. Structural analysis of the Lactobacillus rhamnosus strain KL37C exopolysaccharide.

    Carbohydrate Research 338(7):605 (2003) PMID 12644373

    The exopolysaccharide from the lactic acid bacterium Lactobacillus rhamnosus strain KL37C isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. The polysaccharide mat...