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  1. Full assignment of the 1H and 13C spectra and revision of the O-acetylation site of the capsular polysaccharide of Streptococcus pneumoniae Type 33F, a component of the current pneumococcal polysaccharide vaccine.

    Carbohydrate Research 341(1):68 (2006) PMID 16297895

    The structure of the capsular polysaccharide from Streptococcus pneumoniae Type 33F was originally determined by a combination of chemical methods and limited use of NMR spectroscopy [Can. J. Biochem. Cell Biol.1984, 62, 666-677]. We report full 1H and 13C assignments and confirm the structure o...
  2. Full assignment of the1H and13C spectra and revision of the O-acetylation site of the capsular polysaccharide ofStreptococcus pneumoniaeType 33F, a component of the current pneumococcal polysaccharide vaccine

    Carbohydrate Research 341(1):68 (2006) PMID 16297895

    Full NMR assignments and a revised location for the O-acetyl group in the capsular polysaccharide from Streptococcus pneumoniae Type 33F are reported. The O-acetyl group is attached to O-2 of the →5)-β- d-Gal f residue, rather than the →3)-β- d-...
  3. Full NMR assignment and revised structure for the capsular polysaccharide from Streptococcus pneumoniae type 15B.

    Carbohydrate Research 340(3):403 (2005) PMID 15680595

    The capsular polysaccharide from Streptococcus pneumoniae Type 15B is a component of the 23-valent polysaccharide vaccine against pneumococcal disease. We report full NMR assignments for the native and de-O-acetylated polysaccharide, and confirm that the phosphorylated substituent is glycerol-2-...
  4. Full NMR assignment and revised structure for the capsular polysaccharide fromStreptococcus pneumoniaetype 15B

    Carbohydrate Research 340(3):403 (2005)

  5. Detection of O-acetylated Vi polysaccharide of Salmonella enterica subspecies typhi by enzyme immunoassay.

    Biologicals 32(1):11 (2004) PMID 15026021

    Immunisation with capsular Vi polysaccharide (Vi PS) of Salmonella enterica serovar Typhi (S. typhi) protects against typhoid. This protection depends on the presence of O-acetyl groups on the Vi PS, which form an immunodominant epitope. An antiserum raised against conjugated Vi PS was used as t...
  6. Detection ofO-acetylated Vi polysaccharide ofSalmonella entericasubspeciestyphiby Enzyme Immunoassay

    Biologicals 32(1):11 (2004)

    Immunisation with capsular Vi polysaccharide (Vi PS) of Salmonella enterica serovar Typhi ( S. typhi) protects against typhoid. This protection depends on the presence of O-acetyl groups on the Vi PS, which form an immunodominant epitope. An antiserum raised against conj...
  7. Structural analysis of the Lactobacillus rhamnosus strain KL37C exopolysaccharide.

    Carbohydrate Research 338(7):605 (2003) PMID 12644373

    The exopolysaccharide from the lactic acid bacterium Lactobacillus rhamnosus strain KL37C isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. The polysaccharide mat...
  8. Structural analysis of theLactobacillus rhamnosusstrain KL37C exopolysaccharide

    Carbohydrate Research 338(7):605 (2003)

    The exopolysaccharide from the lactic acid bacterium Lactobacillus rhamnosus strain KL37C isolated from human intestinal flora was prepared by sonication of bacterial cell mass suspended in water followed by centrifugation and cold ethanol precipitation of the supernatant. The polysa...
  9. Use and validation of NMR assays for the identity and O-acetyl content of capsular polysaccharides from Neisseria meningitidis used in vaccine manufacture.

    Journal of Pharmaceutical and Biomedical Analysis 30(4):1233 (2002) PMID 12408914

    We describe a validated NMR (nuclear magnetic resonance) spectroscopic assay for the identity of the capsular polysaccharides (CPSs) from Neisseria meningitidis Groups A, C, W135 and Y used in vaccine manufacture, and to determine the proportion of residues carrying an O-acetyl substituent. Proo...
  10. Physico-chemical and immunological examination of the thermal stability of tetanus toxoid conjugate vaccines.

    Vaccine 20(29-30):3509 (2002) PMID 12297396

    The thermal stability of meningococcal C (MenC)- and Haemophilus influenzae b (Hib)-tetanus toxoid (TT) conjugate vaccines was investigated using spectroscopic and chromatographic techniques and immunogenicity assays in animal models. In this stability study, both the bulk concentrate and final ...
  11. Physico-chemical and immunological examination of the thermal stability of tetanus toxoid conjugate vaccines

    Vaccine 20(29):3509 (2002)

    The thermal stability of meningococcal C (MenC)– and Haemophilus influenzae b (Hib)–tetanus toxoid (TT) conjugate vaccines was investigated using spectroscopic and chromatographic techniques and immunogenicity assays in animal models. In this stability study, both the bulk concentrat...
  12. Use and validation of NMR assays for the identity andO-acetyl content of capsular polysaccharides fromNeisseria meningitidisused in vaccine manufacture

    Journal of Pharmaceutical and Biomedical Analysis 30(4):1233 (2002)

    We describe a validated NMR (nuclear magnetic resonance) spectroscopic assay for the identity of the capsular polysaccharides (CPSs) from Neisseria meningitidis Groups A, C, W135 and Y used in vaccine manufacture, and to determine the proportion of residues carrying an O-acety...
  13. Effect of physico-chemical modification on the immunogenicity ofHaemophilus influenzaetype b oligosaccharide–CRM197conjugate vaccines

    Vaccine 19(23-24):3189 (2001)

    Haemophilus influenzae type b (Hib) poly-ribosyl-ribityl phosphate (PRP) oligosaccharide–CRM197 conjugate vaccines from two different manufacturers (Hib A and Hib B) were subjected to adverse storage conditions and used to establish correlates between physico-chemical characteristics a...
  14. An NMR Spectroscopic Identity Test for the Control of the Capsular Polysaccharide from Haemophilus influenzae Type b

    Biologicals 28(3):175 (2000)

    We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide [called poly(ribosylribitolphosphate) or PRP] from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib polysacchar...
  15. Recombinant Human Albumin as a Stabilizer for Biological Materials and for the Preparation of International Reference Reagents

    Biologicals 26(4):331 (1998)

    Recombinant human albumin expressed inSaccharomyces cerevisiaewas compared with native human serum albumin in its physicochemical properties and in its use as a stabilizer in lyophilized preparations of thyroid-stimulating hormone (TSH), interleukin 15 (IL-15) and granulocyte colony-st...
  16. Full1H NMR assignment and detailedO-acetylation patterns of capsular polysaccharides fromNeisseria meningitidisused in vaccine production

    Carbohydrate Research 296(1):83 (1996)

    We report essentially complete 1H NMR assignments for the capsular polysaccharides from Neisseria meningitidis serotypes A, C, W-135, and Y. These polysaccharides are components of current polysaccharide vaccines against meningococcal infection and of the polysaccharide-protei...