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Roquin Promotes Constitutive mRNA Decay via a Conserved Class of Stem-Loop Recognition Motifs.
Cell 153(4):869-81 (2013) PMID 23663784
We demonstrate that the CDE folds into an RNA stem-loop motif that is specifically recognized by Roquin and Roquin2. Binding of Roquin initiates degradation of TNF-α mRNA and limits TNF-α production in macrophages. Roquin proteins promote mRNA degradation by recruiting the Ccr4-Caf1-Not deadenylase... -
Roquin Promotes Constitutive mRNA Decay via a Conserved Class of Stem-Loop Recognition Motifs.
Cell 153(4):869-81 (2013) PMID 23663784
We demonstrate that the CDE folds into an RNA stem-loop motif that is specifically recognized by Roquin and Roquin2. Binding of Roquin initiates degradation of TNF-α mRNA and limits TNF-α production in macrophages. Roquin proteins promote mRNA degradation by recruiting the Ccr4-Caf1-Not deadenylase... -
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering.
Cell (2013) PMID 23643243
We demonstrate that CRISPR/Cas-mediated gene editing allows the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty - 8 alleles) in mouse embryonic stem (ES) cells with high efficiency. Coinjection of Cas9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes generated mic... -
Cyclic [G(2',5')pA(3',5')p] Is the Metazoan Second Messenger Produced by DNA-Activated Cyclic GMP-AMP Synthase.
Cell (2013) PMID 23647843
We combined structural, chemical, biochemical, and cellular assays to demonstrate that this second messenger contains G(2',5')pA and A(3',5')pG phosphodiester linkages, designated c[G(2',5')pA(3',5')p]. We show that, upon dsDNA binding, cGAS is activated through conformational transitions, resulting... -
Specified neural progenitors sort to form sharp domains after noisy shh signaling.
Cell 153(3):550-61 (2013) PMID 23622240
We analyzed specification patterns and movement trajectories of neural progenitors. We found that specified progenitors of different fates are spatially mixed following heterogeneous Sonic Hedgehog signaling responses. Cell sorting then rearranges them into sharply bordered domains. Ectopically indu... -
Sorting sloppy sonic.
Cell 153(3):509-10 (2013) PMID 23622235
In the classic picture of morphogen-mediated patterning, cells acquire the correct spatial arrangement of specified fates by reading a precisely distributed gradient of morphogen. Xiong et al. now provide evidence for an alternate strategy-cells of the zebrafish neural tube actively... -
Trnp1 regulates expansion and folding of the Mammalian cerebral cortex by control of radial glial fate.
Cell 153(3):535-49 (2013) PMID 23622239
We identified the DNA-associated protein Trnp1 as a regulator of cerebral cortex expansion in both of these dimensions. Gain- and loss-of-function experiments in the mouse cerebral cortex in vivo demonstrate that high Trnp1 levels promote neural stem cell self-renewal and tangential expansion. In c... -
On Your MARK, Get SET(D2), Go! H3K36me3 Primes DNA Mismatch Repair.
Cell 153(3):513-5 (2013) PMID 23622237
Trimethylation of histone H3 on Lys36 (H3K36me3) by SETD2 is linked to actively transcribed regions. Li et al. identify a novel role for H3K36me3 that facilitates DNA mismatch repair (MMR) in cells by targeting the MMR machinery to chromatin during the cell cycle, thereby explaining... -
Four-Dimensional Imaging of E. coli Nucleoid Organization and Dynamics in Living Cells.
Cell (2013) PMID 23623305
Visualization of living E. coli nucleoids, defined by HupA-mCherry, reveals a discrete, dynamic helical ellipsoid. Three basic features emerge. (1) Nucleoid density coalesces into longitudinal bundles, giving a stiff, low-DNA-density ellipsoid. (2) This ellipsoid is radially confine... -
SnapShot: Axon Guidance II.
Cell 153(3):722-722.e1 (2013) PMID 23622251
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