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Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples.
Proc Natl Acad Sci U S A (2013) PMID 23716683
We have found that isolation of pathogenic clones of V. cholerae from surface waters in Bangladesh is dramatically improved by using enrichment media containing autoinducers either expressed from cloned synthase genes or prepared by chemical synthesis. These results may contribute to averting future... -
General mechanism for modulating immunoglobulin effector function.
Proc Natl Acad Sci U S A (2013) PMID 23697368
We demonstrate that sialylation induces significant structural alterations in the Cγ2 domain and propose a model that explains the observed changes in ligand specificity and biological activity. By analogy to related complexes formed by IgE and its evolutionarily related Fc receptors, we conclude th... -
Methionine oxidation activates a transcription factor in response to oxidative stress.
Proc Natl Acad Sci U S A (2013) PMID 23690622
We unravel that HypT is activated by methionine oxidation to methionine sulfoxide. Interestingly, so far only inactivation of cellular proteins by methionine oxidation has been reported. Mutational analysis revealed three methionines that are essential to confer HOCl resistance. Their simultaneous s... -
Biopolymer-reinforced synthetic granular nanocomposites for affordable point-of-use water purification.
Proc Natl Acad Sci U S A (2013) PMID 23650396
We show that such constant release materials can be synthesized in a simple and effective fashion in water itself without the use of electrical power. The nanocomposite exhibits river sand-like properties, such as higher shear strength in loose and wet forms. These materials have been used to develo... -
Kif7 is required for the patterning and differentiation of the diaphragm in a model of syndromic congenital diaphragmatic hernia.
Proc Natl Acad Sci U S A (2013) PMID 23650387
We performed a forward genetic screen in mice to identify unique genes, models, and mechanisms of abnormal diaphragm development. We identified a mutant allele of kinesin family member 7 (Kif7), the disorganized diaphragm (dd). Embryos homozygous for the dd allele possess communicating diaphragmatic... -
Mechanism for nitrogen isotope fractionation during ammonium assimilation by Escherichia coli K12.
Proc Natl Acad Sci U S A (2013) PMID 23650377
We examined a wild-type (WT) strain of Escherichia coli K12 and mutant strains with lesions affecting ammonium-assimilatory proteins. We used isotope ratio mass spectrometry (MS) to assess the nitrogen isotopic composition of cell material when the strains were grown in batch culture at either high... -
WRKY8 transcription factor functions in the TMV-cg defense response by mediating both abscisic acid and ethylene signaling in Arabidopsis.
Proc Natl Acad Sci U S A (2013) PMID 23650359
We report that Arabidopsis thaliana WRKY DNA-binding protein 8 (WRKY8) has a role in mediating the long-distance movement of crucifer-infecting tobacco mosaic virus (TMV-cg). The expression of WRKY8 was inhibited by TMV-cg infection, and mutation of WRKY8 accelerated the accumulation of TMV-cg in sy... -
Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagation.
Proc Natl Acad Sci U S A (2013) PMID 23650383
We identified that Arabidopsis thaliana isoform CPK5 of the plant calcium-dependent protein kinase family becomes rapidly biochemically activated in response to pathogen-associated molecular pattern (PAMP) stimulation. CPK5 signaling resulted in enhanced salicylic acid-mediated resistance to the bac... -
Parkin overexpression during aging reduces proteotoxicity, alters mitochondrial dynamics, and extends lifespan.
Proc Natl Acad Sci U S A (2013) PMID 23650379
We show that ubiquitous or neuron-specific up-regulation of Parkin, in adult Drosophila melanogaster, increases both mean and maximum lifespan without reducing reproductive output, physical activity, or food intake. Long-lived Parkin-overexpressing flies display an increase in K48-linked polyubiquit...
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