Pioneering a Nursing Home Quality Improvement Learning Collaborative: A Case Study of Method and Lessons Learned.
Journal of the American Medical Directors Assoc... 17(2):136 (2016)
To describe the development of a nursing home (NH) quality improvement learning collaborative (QILC) that provides Lean Six Sigma (LSS) training and infrastructure support for quality assurance performance improvement change efforts.
Twenty-seven NHs located in the Greater Rochester...
A Zebrafish Drug-Repurposing Screen Reveals sGC-Dependent and sGC-Independent Pro-Inflammatory Activities of Nitric Oxide.
PLoS ONE 10(10):e0137286 (2015)
Tissue injury and infection trigger innate immune responses. However, dysregulation may result in chronic inflammation and is commonly treated with corticosteroids and non-steroidal anti-inflammatory drugs. Unfortunately, long-term administration of both therapeutic classes can cause unwanted si...
Generation of orientation tools for automated zebrafish screening assays using desktop 3D printing.
BMC Biotechnology 14:36 (2014)
The zebrafish has been established as the main vertebrate model system for whole organism screening applications. However, the lack of consistent positioning of zebrafish embryos within wells of microtiter plates remains an obstacle for the comparative analysis of images acquired in automated sc...
Development of an automated imaging pipeline for the analysis of the zebrafish larval kidney.
PLoS ONE 8(12):e82137 (2013)
The analysis of kidney malformation caused by environmental influences during nephrogenesis or by hereditary nephropathies requires animal models allowing the in vivo observation of developmental processes. The zebrafish has emerged as a useful model system for the analysis of vertebrate organ d...
Behavioral barcoding in the cloud: embracing data-intensive digital phenotyping in neuropharmacology.
Trends in Biotechnology 30(8):421 (2012)
For decades, studying the behavioral effects of individual drugs and genetic mutations has been at the heart of efforts to understand and treat nervous system disorders. High-throughput technologies adapted from other disciplines (e.g., high-throughput chemical screening, genomics) are changing ...
Role and interaction of p53, BAX and the stress-activated protein kinases p38 and JNK in benzo(a)pyrene-diolepoxide induced apoptosis in human colon carcinoma cells.
Archives of Toxicology 86(2):329 (2012)
Polycyclic aromatic hydrocarbons are ubiquitous environmental pollutants formed during incomplete combustion of organic material. For example benzo[a]pyrene (B[a]P) is a constituent and contaminant of cigarette smoke, automobile exhaust, industrial waste and even food products. B[a]P is carcinog...
Facilitating drug discovery: an automated high-content inflammation assay in zebrafish.
Journal of Visualized Experiments (65):e4203 (2012)
Zebrafish larvae are particularly amenable to whole animal small molecule screens due to their small size and relative ease of manipulation and observation, as well as the fact that compounds can simply be added to the bathing water and are readily absorbed when administered in a <1% DMSO soluti...
Superhydrophobic-superhydrophilic micropatterning: towards genome-on-a-chip cell microarrays.
Angewandte Chemie. International edition in Eng... 50(36):8424 (2011)
'Sciencenet'--towards a global search and share engine for all scientific knowledge.
Modern biological experiments create vast amounts of data which are geographically distributed. These datasets consist of petabytes of raw data and billions of documents. Yet to the best of our knowledge, a search engine technology that searches and cross-links all different data types in life s...
Automated feature detection and imaging for high-resolution screening of zebrafish embryos.
BioTechniques 50(5):319 (2011)
The development of automated microscopy platforms has enabled large-scale observation of biological processes, thereby complementing genome scale biochemical techniques. However, commercially available systems are restricted either by fixed-field-of-views, leading to potential omission of featur...
Image-based fluidic sorting system for automated Zebrafish egg sorting into multiwell plates.
Journal of Laboratory Automation 16(2):105 (2011)
The global demand for the reduction of animal testing has led to the emergence of Zebrafish eggs/larvae as model organisms to replace current adult animal testing in, for example, toxicity testing. Because of the egg size (diameter 1.6mm) and the relatively easy maintenance of Zebrafish farms th...
Micropilot: automation of fluorescence microscopy-based imaging for systems biology.
Nature Methods 8(3):246 (2011)
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse...
Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes.
Nature 464(7289):721 (2010)
Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-la...
A high-throughput chemically induced inflammation assay in zebrafish.
BMC Biology 8:151 (2010)
Studies on innate immunity have benefited from the introduction of zebrafish as a model system. Transgenic fish expressing fluorescent proteins in leukocyte populations allow direct, quantitative visualization of an inflammatory response in vivo. It has been proposed that this animal model can b...
Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos.
Nature Methods 6(12):911 (2009)
Zebrafish embryos offer a unique combination of high-throughput capabilities and the complexity of the vertebrate animal for a variety of phenotypic screening applications. However, there is a need for automation of imaging technologies to exploit the potential of the transparent embryo. Here we...
Zebrafish embryos as models for embryotoxic and teratological effects of chemicals.
Reproductive Toxicology 28(2):245 (2009)
The experimental virtues of the zebrafish embryo such as small size, development outside of the mother, cheap maintenance of the adult made the zebrafish an excellent model for phenotypic genetic and more recently also chemical screens. The availability of a genome sequence and several thousand ...
Urban-rural differences in the effect of a Medicare health promotion and disease self-management program on physical function and health care expenditures.
Gerontologist 49(3):407 (2009)
To evaluate the impact of a multicomponent health promotion and disease self-management intervention on physical function and health care expenditures among Medicare beneficiaries. To determine if these outcomes vary by urban or rural residence.
We analyzed data from a 22-month randomized contro...
Novel cargo-binding site in the beta and delta subunits of coatomer.
Journal of Cell Biology 179(2):209 (2007)
Arginine (R)-based ER localization signals are sorting motifs that confer transient ER localization to unassembled subunits of multimeric membrane proteins. The COPI vesicle coat binds R-based signals but the molecular details remain unknown. Here, we use reporter membrane proteins based on the ...
Meeting report: trends and challenges in high content analysis.
Biotechnology Journal 2(8):938 (2007)
An RNAi screening platform to identify secretion machinery in mammalian cells.
Journal of Biotechnology 129(2):352 (2007)
Integrative approaches to study protein function in a cellular context are a vital aspect of understanding human disease. Genome sequencing projects provide the basic catalogue of information with which to unravel gene function, but more systematic applications of this resource are now necessary...